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Reagents & materials

DAPI, Dienes, Giemsa and FISH for phytoplasma research - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to Phytoplasma DAPI/Dienes

Plant health seed testing & plant research · General

DAPI, Dienes, Giemsa and FISH for phytoplasma research

Phloem localisation and morphological support

Reagents & stains

ItemProcure
DAPI and compatible mountant. Dienes method: authenticated Dienes stain. Giemsa method: Giemsa and buffer. FISH method: labelled target probes and hybridisation/wash reagentsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional individual DAPIPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Giemsa or target FISH reagentsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
No combined phytoplasma kit establishedPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Clean glass slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CoverslipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Disposable pipettes or tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Labelled specimen tubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Absorbent paper. Plant sectioning suppliesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Brightfield microscopePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Calibrated pipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Staining rackPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Jars. Fluorescence optics for DAPI/FISHPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Hybridisation equipment for FISHPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Fixed reference tissuePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Healthy tissuePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Probe/no-probe controls as applicablePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Limited): PM 7/133 is correctly titled Generic detection of phytoplasmas. Its existence is not evidence that all these stains are accepted confirmatory tests.

Procurement note: Separate research approaches. A dye signal alone does not provide species confirmation. Dienes composition is source dependent.

Sources:G061 EPPO

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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