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Reagents & materials

Acid fuchsin, cotton blue, McBryde and rose bengal - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

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Acid fuchsin, cotton blue, McBryde and rose bengal

Nematode visualisation in roots or preparations

Reagents & stains

ItemProcure
Acid fuchsinPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Acetic acidPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Sodium hypochlorite clearing reagentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Glycerol/HCl mountant. Cotton-bluePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
McBrydePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Rose-bengal options require their separately authenticated stainPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
MountantPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional prepared nematode/root-staining reagentsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
No single combined kit establishedPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Root dishesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
SlidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CoverslipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
ForcepsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Glass platesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Suitable waste containersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Stereo/brightfield microscopePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Approved heating apparatusPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Chemical ventilationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Fixed nematode/root reference preparationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Unstained root materialPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Limited): EPPO PM 7/119 addresses extraction. A separate staining SOP is needed; an extraction standard does not validate all associated dyes.

Procurement note: UC Davis supports the acid-fuchsin option. McBryde formulation was not authenticated here; obtain the exact controlled method before procuring its constituents.

Sources:G061 EPPO

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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