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Reagents & materials

Nile red, DAPI and Hoechst in polymer studies - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

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Chemical & analytical laboratories · General

Nile red, DAPI and Hoechst in polymer studies

Candidate microplastics and associated cells or biofilms

Reagents & stains

ItemProcure
Nile red with a validated carrier. Associated-cell imaging: DAPI OR a specified Hoechst dye with compatible buffer/fixative. These have different targetsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional individual fluorophore reagent or particle/cell imaging setPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
No generic microplastic-identification kit is impliedPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Low-background filters or glass substratesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Glass sample vesselsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Slides/coverslipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Contamination-controlled sampling consumablesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Fluorescence microscope with dye-matched filtersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Image analysisPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Access to micro-FTIR/Raman if polymer identity is requiredPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Procedural/filter blanksPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Unstained samplePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Polymer referencesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Biological positivePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
No-dye controls for cell imagingPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Research): ISO 24187 establishes analysis principles, not universal validation of these stains. Dye fluorescence alone must not be represented as definitive polymer identification.

Procurement note: ISO 24187 supplies principles only; listed consumables are typical planning inferences. DAPI/Hoechst fluorescence does not identify polymer chemistry.

Sources:G068 ISO

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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