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Reagents & materials

Hoechst or other validated DNA fluorescence staining - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to Hoechst Mycoplasma Screen

Pharmaceuticals biopharma cell therapies & medical devices · General

Hoechst or other validated DNA fluorescence staining

Cell substrate contamination screening

Reagents & stains

ItemProcure
Hoechst DNA stainPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Compatible balanced salt solutionPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Method-specific fixativePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Fluorescence mounting mediumPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reagent waterPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Sigma MYC1 Mycoplasma Stain KitPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Containing Hoechst stainPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
HBSSPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Mounting mediumPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Control slides. Research-use productPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Slides or chamber slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CoverslipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Staining jarsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Pipette tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Light-protective containersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Fluorescence microscope with filters suitable for the selected Hoechst dyePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Required reagent storagePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Fixed positivePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Negative control slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Background controlPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Guidance): FDA cell substrate guidance describes fluorescence based investigation in its defined vaccine context. Apply the current product relevant compendial or validated assay; staining alone is not a universal release test.

Procurement note: The MYC1 example also requires separately sourced fixative. This list covers staining of prepared samples; it is not a cell-culture or product-release protocol.

Sources:G110 US FDA

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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