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Reagents & materials

MTT reduction in reconstructed human epidermis - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to MTT Skin Irritation (RhE)

Cosmetics & personal care · General

MTT reduction in reconstructed human epidermis

Skin irritation hazard identification

Reagents & stains

ItemProcure
MTT concentratePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
DiluentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Model-specific assay mediumPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
DPBSPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Isopropanol/formazan extractantPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Model-specified control reagentsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
A validated reconstructed-human-epidermis system is required. Documented example: EpiDerm EPI-200-SIT plus separately ordered MTT-100 assay kitPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
RhE tissue insertsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Assay platesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Absorbance-reading platesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Sterile pipette tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Blunt forcepsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Wash bottlesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Tissue-rinsing containersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Plate coversPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Plate-reader photometerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Controlled cell-assay incubatorPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Appropriate aseptic work areaPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TimerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Plate shaker if requiredPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Model-specified positive/negative controlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Tissue-lot QCPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Direct-MTT-reductionPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Colour-interference controlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): OECD TG 439 gives a defined tissue test. Address optical or direct MTT interference and apply that model's prediction approach.

Procurement note: MTT reagent alone is not the complete skin-irritation test. Other accepted tissue models have their own validated kit composition and protocol.

Sources:G119 OECD

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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