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Reagents & materials

Gram, Giemsa and spore microscopy; Nosema examination - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

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Agriculture soil microbiology & apiculture · General

Gram, Giemsa and spore microscopy; Nosema examination

Honeybee disease investigation

Reagents & stains

ItemProcure
Gram or Giemsa reagents for the selected preparationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Water/saline for unstained Nosema spore microscopy. Use a named spore stain only when the chosen method specifies itPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional Gram/Giemsa stain setPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
No stain kit is required for routine unstained Nosema microscopyPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Bee tissue preparation tubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Mortar/pestle or validated homogeniser consumablesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
SlidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CoverslipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Counting chamber when enumeratingPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Brightfield microscopePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Dissection toolsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Calibrated pipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Fixed reference spores/slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reagent blankPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Counting referencePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Guidance): Use the relevant WOAH honeybee chapter and validated reference laboratory method. Nosema wet mount examination need not involve a stain, and a spore appearance alone may not resolve species.

Procurement note: Honeybee disease methods differ. Spore morphology alone does not reliably separate Nosema species; extra identification assays are separately specified.

Sources:G003 WOAH

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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