Reagents & materials
Gram, endospore and acid fast staining - Reagents & Materials
What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).
Education biosafety & hygiene monitoring · General
Gram, endospore and acid fast staining
Teaching and supervised practical training
Reagents & stains
| Item | Amount per test | Procure |
|---|---|---|
| Crystal violet, iodine, decolorizer and counterstain. Endospore: selected malachite-green/safranin set. Acid-fast: selected carbol-fuchsin/decolorizer/counterstain set. Reagent water and immersion oilPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Kits
| Item | Amount per test | Procure |
|---|---|---|
| Optional separate teaching stain sets. Do not treat all three methods as one kitPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Consumables & materials
| Item | Amount per test | Procure |
|---|---|---|
| Commercially prepared fixed teaching slides or other approved noninfectious teaching material, slides, racks, tips, labels and absorbent materialPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Equipment
| Item | Amount per test | Procure |
|---|---|---|
| Brightfield microscope, staining station and timer. Method-specific slide heating equipment only where the chosen validated variant requires itPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Controls & reference materials
| Item | Amount per test | Procure |
|---|---|---|
| Prepared teaching/control slides with known staining patternsPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Official basis (Framework): ASM teaching biosafety guidance and the WHO biosafety manual govern risk management. They are not staining recipes or clinical reporting standards.
Procurement note: The biosafety documents govern teaching-lab practice rather than define these reagent formulations. Use the selected stain IFU with approved teaching material.
Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.