Reagents & materials
Bone Marrow Aspirate - Myelogram & M:E Ratio (ICSH) - Reagents & Materials
What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).
Human (Clinical) · Haematology and transfusion
Wright, Wright-Giemsa, May-Grünwald-Giemsa, Leishman and Diff-Quik
Blood and marrow cellular morphology
Reagents & stains
| Item | Amount per test | Procure |
|---|---|---|
| Wright/Wright-GiemsaPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| MGGPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| LeishmanPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Rapid Romanowsky setPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Matched buffer/fixation reagentPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Immersion oilPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Kits
| Item | Amount per test | Procure |
|---|---|---|
| Optional selected Romanowsky setPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Rapid variants have separate eosin/thiazine solutionsPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Consumables & materials
| Item | Amount per test | Procure |
|---|---|---|
| Glass slidesPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Labelled slide boxesPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Transfer pipettesPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Stain jars/racksPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Coverslips if specifiedPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Equipment
| Item | Amount per test | Procure |
|---|---|---|
| Brightfield microscopePer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| PipettesPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| TimerPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Staining stationPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Optional validated blood-film stainerPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Controls & reference materials
| Item | Amount per test | Procure |
|---|---|---|
| Fixed blood/marrow reference filmsPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Stain-lot referencePer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Official basis (Guidance): CLSI H20 third edition 2026 addresses reference differential counting; ICSH 2026 supports validated Romanowsky marrow stains. Classification systems do not replace staining SOPs.
Procurement note: Formulations have distinct buffer/fixation requirements. Reporting/counting guidance does not prescribe a single kit.
Sources:G025 CLSIG026 International Council for Standardization in Haematology
Human (Clinical) · Haematology and transfusion
Perls Prussian blue
Marrow iron and siderotic granules
Reagents & stains
| Item | Amount per test | Procure |
|---|---|---|
| Potassium-ferrocyanide iron reagentPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Matched acidPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Nuclear-red counterstainPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Compatible fixation/mountantPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Kits
| Item | Amount per test | Procure |
|---|---|---|
| Optional Perls/Prussian-blue setPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Consumables & materials
| Item | Amount per test | Procure |
|---|---|---|
| Adhesive slidesPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| CoverslipsPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Tissue cassettes or prepared sectionsPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Staining vesselsPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Equipment
| Item | Amount per test | Procure |
|---|---|---|
| Microtome/tissue-processing access or prepared sectionsPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Brightfield microscopePer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Staining stationPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Chemical ventilationPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Controls & reference materials
| Item | Amount per test | Procure |
|---|---|---|
| Known iron-positive/negative control sections or filmsPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Official basis (Guidance): ICSH 2026 supports Perls reaction. Name the chosen storage iron scale; ring sideroblast quantification is a different assessment.
Procurement note: Documented iron-stain components. Counterstain variants and tissue versus marrow-film preparation differ.
Sources:G026 International Council for Standardization in Haematology
Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.