
Standards library
Official standards behind every STAINIC form
199 official documents and guidelines - ISO, ASTM, OECD, ISTA, WOAH, EPPO, pharmacopoeias, EU law and clinical societies - mapped to the stains and methods that follow them.
Official documentBest practice (no single official standard)
Agriculture soil microbiology & apiculture 7 documents
EPPO PM 7 series (Diagnostic protocols for regulated pests)
IPPC ISPM 27 (Diagnostic protocols)
ISTA International Rules for Seed Testing, Chapter 6 (Biochemical test for viability: the topographical tetrazolium test)
WOAH Manual of Diagnostic Tests and Vaccines for Terrestrial Animals - Nosemosis of honey bees
WOAH Terrestrial Manual - American and European foulbrood
Adam & Duncan 2001, Soil Biol. Biochem. 33 (FDA hydrolysis method)
ISO 23753 (Soil quality - dehydrogenase activity, related enzyme method)
Animal feed & pet food ingredient microscopy 1 documents
Regulation (EC) No 152/2009, Annex VI (Microscopic detection of animal constituents in feed)
Aquaculture & fisheries diagnostics 2 documents
WOAH Manual of Diagnostic Tests for Aquatic Animals
WOAH Manual of Diagnostic Tests for Aquatic Animals - Infection with Perkinsus marinus / P. olseni (RFTM)
Chemical & analytical laboratories 8 documents
Commission Decision 2005/402/EC (Sudan dyes in chilli and spices)
Ph. Eur. 2.2.27 (Thin-layer chromatography)
USP <621> Chromatography
ISO
ISO 24187 (Principles for the analysis of microplastics present in the environment)
ISO/IEC 17025 clause 7.2 (method selection and validation)
Manufacturer instructions for use (IFU)
Peer-reviewed published method
Construction materials & civil engineering 11 documents
ASTM C1777 (Rapid assessment of fine aggregate clay content by methylene blue)
ASTM C295 (Petrographic examination of aggregates)
ASTM C837 (Methylene blue index of clay)
ASTM C856 (Petrographic examination of hardened concrete)
BS EN 14630 (Determination of carbonation depth in hardened concrete by the phenolphthalein method)
EN 933-9 (Methylene blue test for fines)
NT BUILD 492 (Chloride migration coefficient - silver nitrate colorimetric)
ISO/IEC 17025 clause 7.2 (method selection and validation)
Manufacturer instructions for use (IFU)
Peer-reviewed published method
US Geological Survey
Cosmetics & personal care 10 documents
ISO 17516 / ISO 21149 (Cosmetics microbiology)
ISO 22716 (Cosmetics GMP)
OECD TG 439 (In vitro skin irritation: reconstructed human epidermis test method)
OECD TG 460 (Fluorescein leakage test method)
OECD TG 492 (Reconstructed human cornea-like epithelium test method)
Regulation (EC) No 1223/2009 (Cosmetic products)
UN GHS Category 2
ISO
ISO 20391-1 (Biotechnology - Cell counting - General guidance)
ISO 20391-2 (Experimental design and statistical analysis)
Cultural heritage conservation & security documents 6 documents
ICAO Doc 9303 (Machine Readable Travel Documents)
BSI
ISO/IEC 17025 clause 7.2 (method selection and validation)
Manufacturer instructions for use (IFU)
Peer-reviewed published method
Smithsonian Museum Conservation Institute
Education biosafety & hygiene monitoring 11 documents
ASME BPE (Spray device coverage test with riboflavin)
Biological Stain Commission certification
CDC Environmental Cleaning Evaluation toolkit
ISO/IEC 17025 (Testing and calibration laboratories)
WHO Guidelines on Hand Hygiene in Health Care (2009)
ASM
CDC
ISO/IEC 17025 clause 7.2 (method selection and validation)
Manufacturer instructions for use (IFU)
Peer-reviewed published method
WHO
Environmental water wastewater & soil testing 24 documents
APHA Standard Methods 4500-S2- D (Sulfide - methylene blue method)
APHA Standard Methods 5540 C
EN 15204 (Phytoplankton counting - Utermohl technique)
EN 16695 (Estimation of phytoplankton biovolume)
Hobbie et al. 1977 (AODC direct count)
ISO 10260 (Measurement of biochemical parameters - spectrometric determination of chlorophyll-a)
ISO 11731 (Enumeration of Legionella)
ISO 15553 (Isolation and identification of Cryptosporidium oocysts and Giardia cysts from water)
ISO 19458 (Sampling for microbiological analysis)
ISO 6222 (Culturable micro-organisms)
ISO 7393-2 (Free and total chlorine - DPD colorimetric method)
ISO 7875-1 (Anionic surfactants - methylene blue spectrometric method)
ISO 9308-1 (E. coli and coliform bacteria - membrane filtration)
SLMB 333.1 (Flow cytometric total cell count, Swiss Food Book)
US EPA Method 1623.1
USGS TWRI Book 3, Chapter A12 (Fluorometric procedures for dye tracing)
ASTM International
Eikelboom - Filamentous organisms observed in activated sludge
European Union
ISO 24187 (Principles for the analysis of microplastics present in the environment)
ISO/IEC 17025 clause 7.2 (method selection and validation)
Jenkins, Richard & Daigger - Manual on causes and control of activated sludge bulking
Manufacturer instructions for use (IFU)
Peer-reviewed published method
Food dairy beverages brewing & honey 27 documents
AOAC 991.31 (Aflatoxins - immunoaffinity column)
Biological Stain Commission certification
Council Directive 2001/110/EC (honey)
Hobbie et al. 1977 (AODC direct count)
International Honey Commission - Harmonised methods of melissopalynology (Von der Ohe et al., Apidologie 2004)
ISO 11816-1 | IDF 155-1 (Alkaline phosphatase - fluorimetric method)
ISO 13366-1 | IDF 148-1 (Somatic cells - microscopic reference method)
ISO 13366-2 | IDF 148-2 (Fluoro-opto-electronic counters)
ISO 21527 (Yeasts and moulds)
ISO 4833-1 (Aerobic colony count)
ISO 6222 (Culturable micro-organisms)
ISO 7218 (Microbiology of the food chain - general requirements)
ISO 9308-1 (E. coli and coliform bacteria - membrane filtration)
ISO/IEC 17025 (Testing and calibration laboratories)
Regulation (EC) No 2073/2005 (microbiological criteria)
Regulation (EC) No 2074/2005 (as amended) pasteurisation criterion
Regulation (EC) No 853/2004 raw milk criteria
Regulation (EU) 2023/915 (maximum levels for contaminants)
SLMB 333.1 (Flow cytometric total cell count, Swiss Food Book)
WOAH Manual of Diagnostic Tests and Vaccines for Terrestrial Animals
Analytica-EBC - Yeast methods
ASBC Methods of Analysis - Yeast
European Brewery Convention / The Brewers of Europe
International Organisation of Vine and Wine
ISO 20391-1 (Biotechnology - Cell counting - General guidance)
ISO 20391-2 (Experimental design and statistical analysis)
National dairy grading standards (e.g. BIS / FSSAI, USPHS historical)
Forensic science & legal investigation 6 documents
AATCC TM20 (Fiber analysis: qualitative)
ISO 1833 (Quantitative chemical analysis of textiles)
ISO 21043 (Forensic sciences)
OSAC / SWGMAT fibre examination guidelines (forensic)
OSAC Registry standards
UK Home Office Fingermark Visualisation Manual
Human (Clinical) 66 documents
2017 International Consensus on ANCA testing (Bossuyt et al.) - antigen-specific immunoassay first, IIF supplementary
ACR synovial fluid classes; EULAR gout 2016 and CPPD 2011 (compensated polarised light microscopy)
ASCO/CAP ER & PR testing guideline (2020); ASCO/CAP HER2 guideline (2018, 2023 update incl. HER2-low/ultralow); International Ki-67 in Breast Cancer Working Group (2021); St Gallen surrogate subtypes
ASM / CLSI M54 - direct microscopy for fungi
ASM Clinical Microbiology Procedures Handbook; WHO STI laboratory manual (2023) - vaginal wet mount, Amsel criteria
ASM Clinical Virology Manual; CLSI M41 (Viral culture); WHO Laboratory Biosafety Manual (4th ed.)
ASM Manual of Clinical Microbiology; CDC STI Treatment Guidelines 2021 (confirm by NAAT); Tzanck smear is not type-specific
ASM Manual of Clinical Microbiology; EBV serological profile interpretation (VCA, EA, EBNA)
ASM Microbe Library standard protocol
Bancroft's - epithelial mucin & Cryptococcus capsule
Bancroft's Theory & Practice of Histological Techniques; IDSA fungal guidance
CAP Cancer Protocols / ICCR datasets; WHO Classification of Tumours (5th ed.)
CDC DPDx / ASM - modified acid-fast for coccidian parasites & Nocardia
CLSI H44-A2 / ICSH supravital reticulocyte counting
CLSI M35-A2 / ASM Clinical Microbiology Procedures; Nugent 1991 scoring
CLSI M54 (2nd ed., 2021) fungal direct microscopy; ASM Clinical Microbiology Procedures Handbook; IDSA / ESCMID-ECMM fungal guidelines
EADV S2k guideline on bullous pemphigoid (2022); epidermal (roof) vs dermal (floor) binding
EADV S2k guideline on pemphigus (2020) and bullous pemphigoid (2022); perilesional biopsy in Michel's medium
ERS guideline for diagnosis of primary ciliary dyskinesia (2017); BEAT-PCD international consensus on ciliary TEM (Shoemark et al., 2020)
ESMO Clinical Practice Guideline - Cancer of Unknown Primary (2023); NCCN Occult Primary; ICCR datasets; CAP cancer protocols
ESPGHAN 2020 coeliac disease guideline; ACG Clinical Guideline: Coeliac Disease (2023); BSG guideline
EULAR/ACR 2019 SLE classification; highly specific confirmatory anti-dsDNA assay
For fluids without a dedicated reporting system (hydrocele, cyst, drain, nipple discharge): descriptive categories; cell block & ancillary studies per ICCR / CAP cytology protocols
Hazelton & Gelderblom, Emerg Infect Dis 2003 (EM for rapid viral diagnosis); Biel & Gelderblom, J Clin Virol; WHO Laboratory Biosafety Manual (4th ed.)
IAC Yokohama System for Reporting Breast Fine Needle Aspiration Biopsy Cytopathology (2020); triple test (clinical, imaging, cytology); BI-RADS 5th ed.
IAIHG simplified criteria for autoimmune hepatitis (2008); EASL Clinical Practice Guidelines: autoimmune hepatitis (2015) and PBC (2017)
ICAP International Consensus on ANA Patterns (anapatterns.org); EULAR/ACR 2019 SLE criteria (ANA >=1:80); ACR position statement on ANA testing
ICSH 2008 bone marrow guideline; Gale iron grading; WHO MDS-RS criteria
ICSH 2014 recommendations for RBC morphology grading; CLSI H20-A2 differential
ICSH bone marrow specimen & report standards (2008); WHO 2022 / EUMNET reticulin (MF-0 to MF-3), collagen and osteosclerosis grading; IMWG 2014; WHO systemic mastocytosis criteria
ICSH guidelines for the standardisation of bone marrow specimens and reports (2008); WHO Haematolymphoid Tumours 5th ed. (2022); ICC 2022; IMWG 2014
ICSH integrated bone marrow report (2008); WHO Haematolymphoid Tumours 5th ed. (2022); ICC 2022; IMWG 2014
IDSA / ESCMID fungal histopathology; WHO Fungal Priority Pathogens
IDSA bacterial meningitis 2004; ESCMID 2016; age-specific CSF reference ranges; traumatic tap correction. No international CSF cytology category system - descriptive reporting
IDSA Cryptococcal disease guideline; WHO 2022 cryptococcal guidelines
Immuno-EM best practice (Griffiths; Tokuyasu cryosection method); LR White / Lowicryl embedding; antibody validation per IHC good practice
International System for Reporting Serous Fluid Cytopathology (ISRSFC 2020, IAC-ASC) - serous fluids only: pleural, peritoneal (ascitic / washing), pericardial; BTS pleural guideline 2023
ISA Nomenclature Committee / Bancroft's - polarized light apple-green birefringence
Larone's Medically Important Fungi (7th ed.) identification criteria
METAVIR / Ishak liver fibrosis staging; AASLD
Renal Pathology Society recommendations for renal biopsy EM; Reynolds lead citrate (1963); glutaraldehyde-osmium processing per ASM / RPS practice
Renal Pathology Society; ISN/RPS lupus nephritis classification (2018 revision); Oxford MEST-C for IgA nephropathy
The Bethesda System for Reporting Cervical Cytology, 3rd ed. (2014)
The Bethesda System for Reporting Thyroid Cytopathology, 3rd ed. (2023); ACR TI-RADS 2017
The Milan System for Reporting Salivary Gland Cytopathology, 2nd ed. (2023) - ASC / IAC; WHO Head & Neck Tumours (5th ed.)
The Paris System for Reporting Urinary Cytology, 2nd ed. (2022) - ASC / IAC
The Sydney System for the Performance, Classification and Reporting of Lymph Node FNA Cytopathology (IAC 2020); WHO Classification of Haematolymphoid Tumours (5th ed.)
Updated Sydney System for gastritis (1996); OLGA staging
WHO / European Consensus bone marrow fibrosis grading (MF-0 to MF-3)
WHO / NLEP - Ridley Bacteriological Index & Morphological Index
WHO / NTEP (RNTCP) AFB smear grading - IUATLD scale
WHO Basic Malaria Microscopy (2010) & Malaria Microscopy QA Manual v2; WHO LD body density
WHO Bench Aids for the Diagnosis of Intestinal Parasites (2019); CDC DPDx; ICMR SOP stool examination
WHO Classification of Haematolymphoid Tumours (5th ed.) - >=3% blast positivity
WHO Classification of Haematolymphoid Tumours, 5th ed. (2022); ICC 2022; Hans algorithm (2004) for DLBCL cell of origin
WHO Classification of Soft Tissue & Bone Tumours, 5th ed. (2020); WHO Skin Tumours, 5th ed.; CAP melanoma & soft tissue protocols
WHO Classification of Tumours / CAP; CMV, HSV, adenovirus, HPV and polyomavirus IHC or ISH confirmation per ASM and CAP practice
WHO HIV guidelines (advanced disease: CD4 <200 cells/uL); NACO India CD4 testing guidelines; CDC/ICSH single-platform counting
WHO Laboratory Manual for Diphtheria (2021)
WHO Laboratory Techniques in Rabies (5th ed., 2018); WHO Expert Consultation on Rabies TRS 1012 (2018)
WHO LED fluorescence microscopy grading (2011) / IUATLD
WHO Reporting System for Lung Cytopathology (IAC-IARC-WHO, 2022); ATS BAL cellular analysis 2012
WHO Reporting System for Pancreaticobiliary Cytopathology (IAC-IARC-WHO, 2022); WHO Digestive System Tumours (5th ed.)
WHO Reporting System for Soft Tissue Cytopathology (IAC-IARC-WHO, 2023); WHO Soft Tissue & Bone Tumours (5th ed.)
WHO-HAEM5 (2022); ICSH guidelines for bone marrow; Bain - Leukaemia Diagnosis (cytochemistry); FAB criteria (historical)
WHO-HAEM5 & ICC 2022 lineage assignment (incl. MPAL); EuroFlow ALOT/AML panels; ICSH-ICCS 2006 consensus
Materials science electronics & nondestructive testing 23 documents
ASTM E112 (Average grain size)
ASTM E1417/E1417M (Liquid penetrant testing)
ASTM E1444/E1444M (Magnetic particle testing)
ASTM E3 (Preparation of metallographic specimens)
ASTM E407 (Microetching metals and alloys)
ASTM E45 (Inclusion content of steel)
ISO 11665-4 (Radon-222 - integrated measurement with passive sampling, track detectors)
ISO 3452-1 (Penetrant testing - General principles)
ISO 9934-1 (Magnetic particle testing - General principles)
ASTM D3860 (Adsorptive capacity of activated carbon by aqueous phase isotherm)
ASTM F1809 (Guide for selection and use of etching solutions to delineate structural defects in silicon, withdrawn - historical reference)
ASTM International
IPC
IPC J-STD-001 (Soldered electrical and electronic assemblies)
IPC-A-610 (Acceptability of electronic assemblies)
ISO
ISO 16700 (SEM image magnification calibration)
ISO 24187 (Principles for the analysis of microplastics present in the environment)
JIS K 1474 (methylene blue adsorption of activated carbon)
OEM fluid and dye specifications
SAE J2297 (UV leak detection dyes for A/C systems)
Sawyer, Grubb & Meyers - Polymer Microscopy (3rd ed.)
SEMI MF1809 / SEMI MF1726
Mining geology petroleum & coal 12 documents
ASTM C1777 (Rapid assessment of fine aggregate clay content by methylene blue)
ASTM C295 (Petrographic examination of aggregates)
ASTM C837 (Methylene blue index of clay)
ASTM C856 (Petrographic examination of hardened concrete)
EN 933-9 (Methylene blue test for fines)
ISO 7404-3 (Coal maceral group analysis)
ISO 7404-4 (Microlithotype analysis)
ISO 7404-5 (Vitrinite reflectance)
USGS TWRI Book 3, Chapter A12 (Fluorometric procedures for dye tracing)
ISO/IEC 17025 clause 7.2 (method selection and validation)
Manufacturer instructions for use (IFU)
Peer-reviewed published method
Packaging & container integrity 2 documents
ASTM F1929 (Seal leaks in porous medical packaging by dye penetration)
ASTM F3039 (Leaks in nonporous packaging or flexible barrier materials by dye penetration)
Pharmaceuticals biopharma cell therapies & medical devices 22 documents
ASME BPE (Spray device coverage test with riboflavin)
ASTM F3039 (Dye penetration, nonporous packaging)
CDC Environmental Cleaning Evaluation toolkit
EU GMP Annex 1 (2022) Manufacture of sterile medicinal products
ICH Q2(R2) (Validation of analytical procedures)
INHAND nomenclature (STP / goRENI)
ISO 10993-5 (Biological evaluation of medical devices - in vitro cytotoxicity)
OECD Principles of Good Laboratory Practice
OECD TG 432 (In vitro 3T3 NRU phototoxicity test)
Ph. Eur. 2.6.12 / 2.6.13
Ph. Eur. 2.7.24 (Flow cytometry)
Ph. Eur. 2.9.20 (Particulate contamination: visible particles)
USP <1027> Flow cytometry
USP <1116> Microbiological control and monitoring of aseptic processing environments
USP <1207> (Package integrity evaluation - sterile products)
USP <1790>
USP <61> / <62> Microbial examination of non-sterile products
USP <790> Visible particulates in injections
WHO Guidelines on Hand Hygiene in Health Care (2009)
ISO
ISO 20391-1 (Biotechnology - Cell counting - General guidance)
ISO 20391-2 (Experimental design and statistical analysis)
Plant health seed testing & plant research 11 documents
AWPA Book of Standards
EN 152 (Blue stain in service - preservative test)
EPPO PM 7 series (Diagnostic protocols for regulated pests)
EPPO PM 7/97 (Indirect immunofluorescence test for plant pathogenic bacteria)
IPPC ISPM 27 (Diagnostic protocols)
ISTA Chapter 19 (Testing species and variety)
ISTA International Rules for Seed Testing, Chapter 6 (Biochemical test for viability: the topographical tetrazolium test)
ISTA International Rules for Seed Testing, Chapter 7 (Seed health testing)
TAPPI T 401 (Fiber analysis of paper and paperboard)
ISO 20391-1 (Biotechnology - Cell counting - General guidance)
ISO 20391-2 (Experimental design and statistical analysis)
Regulatory toxicology & chemical safety laboratories 12 documents
INHAND nomenclature (STP / goRENI)
ISO 10993-5 (Biological evaluation of medical devices - in vitro cytotoxicity)
OECD Principles of Good Laboratory Practice
OECD TG 432 (In vitro 3T3 NRU phototoxicity test)
OECD TG 439 (In vitro skin irritation: reconstructed human epidermis test method)
OECD TG 442E (In vitro skin sensitisation: h-CLAT)
OECD TG 460 (Fluorescein leakage test method)
OECD TG 474 (Mammalian erythrocyte micronucleus test)
OECD TG 487 (In vitro mammalian cell micronucleus test)
OECD TG 489 (In vivo mammalian alkaline comet assay)
OECD TG 492 (Reconstructed human cornea-like epithelium test method)
UN GHS Category 2
Shipping & ballast water treatment testing 3 documents
IMO BWM Convention, Regulation D-2 (Ballast water performance standard)
IMO Guidelines G2 (Ballast water sampling)
ISO 10260 (Measurement of biochemical parameters - spectrometric determination of chlorophyll-a)
Textiles leather dyes & coatings 12 documents
AATCC TM20 (Fiber analysis: qualitative)
EN ISO 14362-1 (Aromatic amines from azo colorants)
ISO 105-A02 (Grey scale for assessing change in colour)
ISO 105-A03 (Grey scale for assessing staining)
ISO 1833 (Quantitative chemical analysis of textiles)
OSAC / SWGMAT fibre examination guidelines (forensic)
REACH Annex XVII, entry 43 (30 mg/kg limit)
ASTM International
ISO
ISO/IEC 17025 clause 7.2 (method selection and validation)
Manufacturer instructions for use (IFU)
Peer-reviewed published method
Veterinary & animal health 13 documents
AAFP Feline Retrovirus Testing and Management Guidelines (2020)
ASVCP QALS (cytology); Camus et al. Vet Pathol 2016 cytological grading of canine mast cell tumours - histopathology remains the reference
ASVCP Quality Assurance & Laboratory Standards (QALS); species-specific reference intervals (ASVCP RI guideline); ACVIM consensus on IMHA (2019)
ASVCP quality assurance guidelines
IETS Manual (International Embryo Technology Society) - embryo evaluation codes
Makris et al. 2009 terminology of developmental abnormalities
OECD TG 414 (Prenatal developmental toxicity) - skeletal examination
Society for Theriogenology Bull Breeding Soundness Evaluation: >=30% progressive motility, >=70% normal morphology and minimum scrotal circumference for age
WAAVP guideline for the faecal egg count reduction test (Kaplan et al. 2023); ESCCAP companion-animal faecal diagnostics
WOAH Manual of Diagnostic Tests and Vaccines for Terrestrial Animals
WOAH Terrestrial Manual - Brucellosis, Q fever and Enzootic abortion of ewes chapters: modified ZN is presumptive; confirm by culture / PCR / serology
WOAH Terrestrial Manual - Paratuberculosis (Johne's disease) and Mammalian tuberculosis chapters: smear is presumptive only; confirm by culture / PCR
WOAH Terrestrial Manual Chapter 3.1.19 Rabies (adopted 2023); WHO Laboratory techniques in rabies, 5th ed. (2018)
Wood pulp & paper 15 documents
AWPA Book of Standards
AWPA Book of Standards - penetration indicator methods for copper and boron
AWPA U1 (Use Category System)
EN 152 (Blue stain in service - preservative test)
ISO 2470-1 (Diffuse blue reflectance factor - indoor daylight, ISO brightness)
ISO 2470-2 (D65 brightness)
ISO 9184-1 (Paper, board and pulps - Fibre furnish analysis - General method)
ISO 9184-3 (Herzberg staining test)
ISO 9184-4 (Graff C staining test)
ISO 9184-5 (Lofton-Merritt staining test)
TAPPI T 401 (Fiber analysis of paper and paperboard)
ISO
ISO/IEC 17025 clause 7.2 (method selection and validation)
Manufacturer instructions for use (IFU)
Peer-reviewed published method
STAINIC summarises the reporting elements of these documents; it does not reproduce them or certify compliance. Always use the current edition, the manufacturer's instructions and your validated SOP.
