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Immunofluorescence

ANCA Immunofluorescence (IIF)

ANCA immunofluorescence on ethanol-fixed neutrophils shows cytoplasmic (C-ANCA) or perinuclear (P-ANCA) patterns. Confirmed with PR3 and MPO antigen tests, it supports the diagnosis of ANCA-associated vasculitis.

Guideline: 2017 International Consensus on ANCA testing (Bossuyt et al.) - antigen-specific immunoassay first, IIF supplementary

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Immunofluorescence

ANCA Immunofluorescence (IIF)

2017 International Consensus on ANCA testing (Bossuyt et al.) - antigen-specific immunoassay first, IIF supplementary

Negative / within normal

Neutrophil Substrate

Impression / Conclusion

Finished reviewing the findings?

Smear Camera — teach STAINIC to read ANCA IIF

Snap the smear, film or microscope field with your phone camera, or upload a microscope camera image. Photos help STAINIC learn stain patterns so it can suggest findings in future.

Mobile data / Wi-Fi

Each photo (1-12 MB) is uploaded over your internet connection. On mobile data this uses your data plan and may incur charges; prefer Wi-Fi for many photos.

How AI will use photos

Photos are kept in a separate STAINIC image library (not your storage quota) to build future machine-learning models that recognise stain patterns and suggest findings. No AI analysis runs on them today and nothing is auto-filled yet.

Disclaimer

Photograph only the smear / film / field — never patient names, labels, faces or documents. Photos are not linked to any patient. Future AI suggestions will be decision-support only and must always be verified by a qualified pathologist; they are not a diagnosis or treatment advice.

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Guideline content, scores and auto-drafted impressions are decision-support and educational aids. They do not replace the professional judgement of a qualified pathologist or microbiologist, who must review and sign every report.

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