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Reagents & materials

Calcofluor White (CFW) Fluorescent Stain - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to Calcofluor White

Human (Clinical) · Mycology

Calcofluor white and other optical brighteners

Fluorescent fungal cell wall detection

Reagents & stains

ItemProcure
Calcofluor whitePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
KOH where part of the selected methodPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Optional kit counterstainPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reagent waterPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional fungal fluorescence setPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Other brighteners require validationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Glass slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Labelled slide boxesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Transfer pipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Stain jars/racksPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Coverslips if specifiedPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Fluorescence microscope with matched filtersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TimerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reagent storage per IFUPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Fixed fungal-positivePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Negative slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Fluorescence QCPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): TP39 describes KOH with calcofluor. Blankophor substitutions require validated conditions; fluorescence is not species specific.

Procurement note: TP39 documents calcofluor categories. Substitute brighteners require their own IFU/filter validation; wet mounts do not automatically need fixation.

Sources:G009 UK Health Security Agency and Royal College of Pathologists

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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