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Reagents & materials

IgA Endomysial Antibody (EMA) IIF - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to EMA IIF

Human (Clinical) · Autoimmune immunofluorescence serology

IgA endomysial antibody immunofluorescence

Selected coeliac disease confirmation

Reagents & stains

ItemProcure
Purchased endomysial substratePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Anti-human IgA fluorescent conjugatePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Matched diluent/washPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
MountantPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Validated IgA endomysial IFA systemPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Verified primate-oesophagus categoryPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Assay substrate slides or prepared sectionsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CoverslipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Dilution tubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Pipette tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Humid chamberPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Fluorescence microscope with matched filtersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TimerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reagent storage per IFUPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
EMA-IgA positive/negative seraPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Tissue controlPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Guidance): ESPGHAN 2020 uses total IgA and tissue transglutaminase IgA initially, with EMA in specified pathways. Remove the unsupported combined anti gliadin IF label.

Procurement note: SC21 verifies substrate/IgA category. Ancillaries remain assay-specific; no anti-gliadin IF reagent inferred.

Sources:G040 ESPGHAN

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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