Reagents & materials

India Ink Preparation - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to India Ink

Human (Clinical) · Mycology

Lactophenol cotton blue; KOH clearing; India ink

Fungal structures and capsule visualization

Reagents & stains

ItemProcure
Lactophenol cotton bluePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
KOH clearing reagentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Microscopy India inkPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reagent waterPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional prepared fungal mount/clearing reagentsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Not a single mixturePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Glass slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Labelled slide boxesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Transfer pipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Stain jars/racksPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Coverslips if specifiedPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Brightfield microscopePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TimerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Staining stationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Fixed fungal morphology control slidePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Negative/background preparationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): TP39 includes these preparations; KOH is a clearing agent rather than a stain. India ink has limited sensitivity and does not replace cryptococcal antigen testing.

Procurement note: Alternative preparations with different uses. Wet mounts need coverslips; no routine permanent fixative/mountant is assumed.

Sources:G009 UK Health Security Agency and Royal College of PathologistsG018 CLSI

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