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Christmas tree stain using nuclear fast red and picroindigocarmine - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

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Forensic science & legal investigation · General

Christmas tree stain using nuclear fast red and picroindigocarmine

Microscopic search for spermatozoa

Reagents & stains

ItemProcure
Nuclear fast red staining reagentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Picroindigocarmine counterstainPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Specified fixativePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Rinse waterPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Mounting mediumPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional validated Christmas-tree sperm stain set containing the two named stainsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Microscope slides/coverslipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Specimen swabs/extraction tubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Staining jars/racksPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Pipette tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Brightfield microscope with suitable high-power objectivesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Slide drying/preparation equipmentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Specimen containment appropriate to forensic biological materialPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Sperm-containing positive slidePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Negative slidePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reagent/background checkPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Microscope calibrationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): Authentic forensic laboratory SOP. Report sperm findings separately from presumptive semen tests; negative microscopy does not exclude seminal material.

Procurement note: Verify formulation against the agency method and supplier IFU. Microscopy supplies support a sperm search and do not establish semen absence or individual identity.

Sources:G077 Indiana State Police

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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