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Reagents & materials

Acid phosphatase and historical Lugol methods - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

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Forensic science & legal investigation · General

Acid phosphatase and historical Lugol methods

Semen screening and legacy methods

Reagents & stains

ItemProcure
Defined phosphate substratePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Diazonium coupling reagentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Acidic bufferPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Commonly an alpha-naphthyl-phosphate/Fast-Blue system. Historical Lugol route: iodine/potassium iodide reagent, separately specifiedPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional validated presumptive acid-phosphatase test kitPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
No current confirmatory semen kit is implied for historical Lugol testingPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Swabs or transfer paperPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Spot-test cards/tubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Slides/coverslips only for the legacy microscopy routePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Visible examinationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Image documentationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Timer where required by controlled methodPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Microscope only for the separate legacy routePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Known positive enzyme/semen referencePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
SubstratePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reagent negativesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Interference controls as requiredPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Limited): Acid phosphatase is presumptive and requires appropriate controls. Lugol-based legacy testing is not established here as a current confirmatory semen method.

Procurement note: Substrate/coupler names are typical families; choose the exact SOP formulation. Historical Lugol supplies are descriptive and are not a recommendation for current casework.

Sources:G077 Indiana State Police

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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