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Gram stain; methylene blue; endospore stains - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to Gram, MB & Spore (Food)

Food dairy beverages brewing & honey · General

Gram stain; methylene blue; endospore stains

Food-isolate morphology and characterisation

Reagents & stains

ItemProcure
Crystal violet, Gram iodine, method-matched decolorizer, safranin or specified counterstain, reagent water, immersion oil. Methylene-blue method: methylene blue. Endospore method: malachite green and Per validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional separate GramPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Simple-stainPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Endospore staining setsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Clean glass slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CoverslipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Disposable pipettes or tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Labelled specimen tubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Absorbent paperPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Brightfield microscopePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Calibrated pipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Staining rack and jars. Controlled heating device only for methods requiring itPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Fixed Gram-positive/negativePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Spore reference slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Guidance): FDA BAM R32 authenticates Gram staining. FDA lists methylene-blue reagents; the target chapter must justify their use. No universal food-spore-stain endorsement follows from ISO 7218.

Procurement note: FDA confirms Gram and named BAM reagents. Food matrix and target identification requirements remain separate.

Sources:G095 US Food and Drug AdministrationG096 US Food and Drug AdministrationG097 ISO

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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