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Reagents & materials

Methylene blue; methylene violet - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to Methylene Blue Yeast Viability

Food dairy beverages brewing & honey · General

Methylene blue; methylene violet

Brewing-yeast viability estimation

Reagents & stains

ItemProcure
Either methylene blue or methylene violet with the selected yeast-test diluent/bufferPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Purified waterPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional prepared brewing-yeast viability stainPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Dyes are alternativesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Haemocytometer/counting chamberPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CoverslipPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Sample tubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Pipette tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Brightfield microscopePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Calibrated pipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Tally counterPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Assay-qualified reference yeast preparationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Unstained controlPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): Analytica EBC 3.2.1.1 explicitly names both dyes. OIV also describes methylene-blue yeast assessment. Staining results should not be assumed equivalent to reproductive viability measured by culture.

Procurement note: EBC authenticates both stain names. Final buffer and interpretation follow the selected EBC/OIV method; staining does not directly measure colony formation.

Sources:G104 European Brewery Convention / The Brewers of EuropeG105 International Organisation of Vine and Wine

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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