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Trypan blue and acridine orange with propidium iodide - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

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Pharmaceuticals biopharma cell therapies & medical devices · General

Trypan blue and acridine orange with propidium iodide

Cell concentration and viability estimates

Reagents & stains

ItemProcure
Trypan blue and isotonic diluent. Fluorescence option: acridine orange plus propidium iodide and the validated sample bufferPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional trypan-blue reagent or AO/PI viability reagent set. Documented example: ReadyCount Green/Red Viability Stain, for a compatible counterPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
HaemocytometerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Coverslip or instrument-specific counting slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Sample tubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Pipette tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Brightfield microscope/manual counting chamber for trypan blue, or a fluorescence cell counter with compatible AO/PI channelsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Qualified cell-count reference materialPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Viable/nonviable cell-control preparationsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Instrument checksPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Framework): ISO 20391-1:2026 provides the current counting framework. Validate the dye, sample, instrument and intended measurand; membrane integrity does not establish product potency.

Procurement note: These are alternative measurement routes. The instrument example is research-use documentation, not authorization for a cell-therapy release assay.

Sources:G109 ISO

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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