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Reagents & materials

MTT reduction in reconstructed corneal epithelium - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to MTT Eye Irritation (RhCE)

Cosmetics & personal care · General

MTT reduction in reconstructed corneal epithelium

Identification of chemicals not requiring eye hazard classification

Reagents & stains

ItemProcure
MTT concentrate/diluentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
RhCE assay mediumPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Calcium-Per validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Magnesium-free DPBSPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Isopropanol/formazan extractantPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Sterile tissue-culture-grade deionized waterPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Methyl acetate for the named EpiOcular controlPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
A validated RhCE system is required. Documented example: EpiOcular OCL-200-EIT plus separately ordered MTT-100 assay kitPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Corneal-epithelium tissue insertsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Assay platesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Absorbance-reading platesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Sterile/wide-bore tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
ForcepsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Rinsing vesselsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Plate coversPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Plate-reader photometerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Controlled cell-assay incubatorPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Aseptic work areaPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Calibrated pipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TimerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Plate shakerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Supplied methyl-acetate positive controlPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Separately required sterile-water negative controlPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Tissue-lot QCPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
MTT/colour-interference controlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): OECD TG 492 identifies chemicals not requiring classification for eye irritation or serious eye damage (UN GHS No Category), within its defined domain. It does not establish every eye hazard category.

Procurement note: Use the EIT protocol for the stated TG 492 purpose. The supplier also offers other ocular assays; they are not interchangeable with this endpoint.

Sources:G120 OECD

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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