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Reagents & materials

DAPI and acridine orange epifluorescence - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to DAPI / AO Direct Count

Environmental water wastewater & soil testing · General

DAPI and acridine orange epifluorescence

Direct total microbial counts

Reagents & stains

ItemProcure
DAPI or acridine orangePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Particle-free bufferPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Selected fixative when requiredPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Low-background mounting mediumPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional prepared direct-count fluorochrome reagentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
No kit universally requiredPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Black low-autofluorescence membrane filtersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
SlidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CoverslipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Filter supportPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Filtered tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Epifluorescence microscope with matching filtersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Vacuum filtration manifoldPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Calibrated pipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Filter/reagent blankPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reference particle/cell materialPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Replicate count checkPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Guidance): Standard Methods 9216 concerns direct total microbial counting. Establish the selected dye procedure and reporting basis from the licensed method; total counts are not colony forming units.

Procurement note: Public Standard Methods scope supports direct counts. Complete reagent and filter specifications need the controlled method.

Sources:G124 APHA AWWA WEF

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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