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Fluorescein diacetate and CMFDA vital fluorescence - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to FDA / CMFDA Protist Viability

Shipping & ballast water treatment testing · General

Fluorescein diacetate and CMFDA vital fluorescence

Living protist assessment in the 10 to less than 50 micrometre size class

Reagents & stains

ItemProcure
Fluorescein diacetatePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CMFDAPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Manufacturer-specified stock solventPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Test-plan-compatible dilution water/bufferPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Individual FDA/CMFDA reagents or a validated dual-stain supply package. No universal approved ballast-water kit is impliedPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Appropriate sample bottlesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Counting chambersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Pipette tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Size-calibration slidePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Dark reagent containersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Epifluorescence microscope with appropriate green-fluorescence opticsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Calibrated counting volumePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Size measurement capabilityPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Reagent/unstained blanksPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Instrument standardsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Test-plan-qualified viability reference materialPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): EPA ETV recommends dual FDA/CMFDA microscopy with on-site validation and notes limitations for cysts. Follow the authorised test plan; 46 CFR 162.060-5 incorporates the protocol for specified US testing.

Procurement note: Applies to the nominal 10 to less than 50 micrometre protist class. Site validation, organism-dependent uptake and cyst limitations remain part of the approved test plan.

Sources:G146 US EPA and US Coast GuardG147 US eCFR

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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