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MTT reduction - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

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Regulatory toxicology & chemical safety laboratories · General

MTT reduction

Skin irritation and eye hazard No Category endpoints

Reagents & stains

ItemProcure
MTTPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Tissue-model diluent/assay mediumPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
DPBSPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Validated formazan extractant. EpiOcular specifically uses calcium-Per validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Magnesium-free DPBSPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Sterile tissue-culture-grade deionized waterPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Methyl-acetate controlPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
RhE or RhCE tissue system appropriate to the endpoint, with its MTT assay reagent set. Examples: EpiDerm EPI-200-SIT or EpiOcular OCL-200-EIT plus MTT-100Per validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Model-specific tissue insertsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Assay platesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Absorbance-reading platesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Sterile tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
ForcepsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Rinsing containersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Plate coversPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Plate-reader photometerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Controlled cell-assay incubatorPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Appropriate aseptic work areaPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TimerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Plate shaker if requiredPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Model-specified positive/negative controlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Tissue-lot QCPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Direct-MTT-reductionPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Colour-interference controlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): OECD TG 439 addresses skin irritation; TG 492 identifies chemicals not requiring eye hazard classification. Different tissue models and prediction rules apply.

Procurement note: Skin and corneal models are distinct systems. Select the endpoint and accepted protocol before ordering. MTT reagent alone is not a complete test.

Sources:G119 OECDG120 OECD

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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