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Reagents & materials

Fluorescein leakage - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to Fluorescein leakage

Regulatory toxicology & chemical safety laboratories · General

Fluorescein leakage

Selected severe eye irritation and corrosion hazards

Reagents & stains

ItemProcure
Sodium fluoresceinPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Calcium-containingPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Phenol-red-free HBSSPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Model-appropriate assay mediumPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Specified control reagentsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
No universal stain kit. Procure the qualified barrier-test reagents and inserts for the selected TG 460 implementationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Qualified MDCK barrier preparationsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Compatible membrane insertsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Receiving platesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Sterile tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Fluorimeter or fluorescence plate reader with appropriate fluorescein opticsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Cell-assay incubatorPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
MicroscopePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Calibrated pipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
NegativePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Positive controlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Cell-free maximum-leakage insertPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Fluorescence blankPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): OECD TG 460 is an in vitro barrier test with an explicit applicability domain. It is not an in vivo ocular surface staining scale.

Procurement note: This is an in vitro barrier assay, not fluorescein-strip examination of a patient eye. The particular membrane insert and cell system are integral components.

Sources:G122 OECD

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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