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Reagents & materials

Giemsa, acridine orange or validated DNA specific fluorescence - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to Micronucleus Staining

Regulatory toxicology & chemical safety laboratories · General

Giemsa, acridine orange or validated DNA specific fluorescence

In vitro micronucleus assessment

Reagents & stains

ItemProcure
Giemsa with bufferPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Acridine orangePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Or another validated DNA-specific dyePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Compatible fixative, wash solution and mountant. Cytochalasin B only for a cytokinesis-block assay variantPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional micronucleus staining reagent set matched to the validated method. No single stain kit supplies the entire TG 487 assayPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Prepared cell samplesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Microscope slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CoverslipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Counting/documentation materialsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Brightfield microscope for Giemsa or fluorescence microscope for fluorescent stainingPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Image-analysis/counting system if validatedPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Qualified positive/negative assay controlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reference slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Staining/background controlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): OECD TG 487 recognises these staining approaches. Nuclear specificity, artefact exclusion and the test's scoring criteria remain essential.

Procurement note: TG 487 supports the stain classes. Exact fixation, cytokinesis-block approach, cell model and kit components depend on the validated assay.

Sources:G148 OECD

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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