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Centromere FISH or anti kinetochore immunolabelling - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to Centromere FISH (Micronuclei)

Regulatory toxicology & chemical safety laboratories · General

Centromere FISH or anti kinetochore immunolabelling

Mechanistic interpretation of micronuclei

Reagents & stains

ItemProcure
Centromeric probePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
HybridisationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Wash buffersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
DNA counterstainPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Antifade mountant. Immunolabelling option: anti-kinetochore antibodyPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Compatible fluorescent secondary antibodyPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
BlockingPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional pan-centromeric FISH probe set or qualified kinetochore-antibody set. These are alternative adjunct methodsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Prepared cell slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CoverslipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Hybridisation seals where requiredPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Light-protective materialsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Multichannel fluorescence microscopePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Controlled hybridisation system for FISH or humid staining chamber for immunolabellingPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Known centromere-positive/negative micronucleus preparationsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Probe or antibody background controlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Guidance): OECD TG 487 describes optional chromosome content assessment. This is an adjunct to the genotoxicity endpoint rather than a separate universal clinical test.

Procurement note: The guideline permits mechanistic follow-up. Probe/antibody specificity, specimen preparation and detection system determine the supply list.

Sources:G148 OECD

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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