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Fluorochrome antibodies to CD54 and CD86 - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to h-CLAT CD54/CD86

Regulatory toxicology & chemical safety laboratories · General

Fluorochrome antibodies to CD54 and CD86

Cell activation endpoints in skin sensitisation assessment

Reagents & stains

ItemProcure
Qualified fluorochrome-labelled anti-CD54Per validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Anti-CD86 antibodiesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Matched isotype controlPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Viability dyePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Staining bufferPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Human-globulin blocking reagent for the specified h-CLAT variantPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Assay-qualified antibody/viability panel. No universal skin-sensitisation staining kit is specifiedPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Qualified assay-cell preparationsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Flow tubes or round-bottom platesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Sterile tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Light-protective coversPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Compatible flow cytometerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Analysis softwarePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CentrifugePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Required cell-assay equipmentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
IsotypePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
UnstainedPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Single-colour controlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Viability controlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Instrument QCPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Method-defined proficiency/positive/negative controlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): OECD TG 442E includes h-CLAT and related defined methods. Apply the selected method and integrated interpretation; these markers are not generic allergy diagnosis tests.

Procurement note: The h-CLAT method specifies antibody characteristics and permits justified equivalents. Other assays within TG 442E use different reagents and must not be conflated.

Sources:G150 OECD

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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